Human CKMT1A antibody

CKMT1A, also known as Creatine kinase, mitochondrial 1A, is 417 amino acid protein. Mitochondrial creatine kinase (MtCK) exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK (CKMT1) . It is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis.

Product Specifications

Product Name Alternative

Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CKMT1A (40-417aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT17A2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK, ATGA0296-10 µg, ATGA0296-20 µg, ATGA0296-50 µg, ATGA0296-100 µg, ATGA0296-250 µg, ATGA0296-500 µg, ATGA0296-1 mg, ATGA0296-10, ATGA0296-20, ATGA0296-50, ATGA0296-100, ATGA0296-250, ATGA0296-500, ATGA0296-1

References & Citations

Haas. R.C., et al. (1989) J Biol Chem 264 (5) : 2890-2897 ; ; Stachowiak. O., et al. (1998) Mol Cell Biochem 184 (1-2) : 141-151; ; Lipskaya. T.Y. (2001) Biochemistry Mosc 66 (10) : 1098-1111

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001015001

Uniprot Accession Number

P12532

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate

FACS Description

Flow cytometry analysis of CKMT1A in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CKMT1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKMT1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .