Human ASNA1 antibody

ASNA1, also as known as ARSA and TRC40, is the human homolog of the bacterial arsA, a member of the ATPase superfamily. ArsA and ArsB have been postulated to form a membrane complex which functions as an anion-translocating. It recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ArsA hydrolyses ATP in the presence of its anionic substrate antimonite and produces resistance to arsenite and antimonite. The active form of ArsA is a homodimer with four nucleotide binding sites, two from each monomer.

Product Specifications

Product Name Alternative

ATPase ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, hASNA-I, TRC40

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ASNA1 (1-348aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2A1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ASNA1, ATGA0293-10 µg, ATGA0293-20 µg, ATGA0293-50 µg, ATGA0293-100 µg, ATGA0293-250 µg, ATGA0293-500 µg, ATGA0293-1 mg, ATGA0293-10, ATGA0293-20, ATGA0293-50, ATGA0293-100, ATGA0293-250, ATGA0293-500, ATGA0293-1

References & Citations

Ota T and Suzuki Y. (2004) Nat genet 36 (1) : 40-5.; ; Kurdi-Haidar B.. (1996) Genomics 36 (3) : 486-91.; ; Stefanovic S. (2007) Cell 128 (6) : 1147-59.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_004308

Uniprot Accession Number

O43681

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASNA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 3.: MCF7 cell lysate

FACS Description

Flow cytometry analysis of ASNA1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ASNA1 in HeLa cells. The cell was stained with ATGA0293 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .