Human DJ-1/PARK7 antibody

Parkinson disease (autosomal recessive, early onset) 7, also known as PARK7/DJ-1, has been shown to interact with EFCAB6 and protein inhibitor of activated STAT2. Defects in PARK7 are the cause of autosomal recessive early-onset Parkinson's disease 7. This protein belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.

Product Specifications

Product Name Alternative

Parkinson disease protein 7, Parkinsonism associated deglycase, parkinson protein 7, Parkinson disease autosomal recessive early onset 7, Maillard deglycase, Oncogene DJ1, Protein DJ-1, DJ-1, DJ1, GATD2, Protein/nucleic acid deglycase DJ-1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Park7/DJ-1 (1-189aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1E12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DJ1, DJ-1, DJ 1, DJ1 protein, GATD2, Maillard deglycase, Oncogene DJ1, PARK7, Parkinson disease autosomal recessive early onset 7, Parkinson disease protein 7, Parkinson disease protein 7 CAP1, parkinson protein 7, Parkinsonism associated deglycase, Protein DJ-1, Protein/nucleic acid deglycase DJ-1, SP22, PARK 7, ATGA0292-10 µg, ATGA0292-20 µg, ATGA0292-50 µg, ATGA0292-100 µg, ATGA0292-250 µg, ATGA0292-500 µg, ATGA0292-1 mg, ATGA0292-010, ATGA0292-020, ATGA0292-050, ATGA0292-100, ATGA0292-250, ATGA0292-500, ATGA0292-01M

References & Citations

Entrez Gene: PARK7; ; Bonifati V., et al. (2003) Science 299 (5604) : 256-259.; ; Takahashi K., et al. (2001) J. Biol. Chem. 276 (40) : 37556-63.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_009193

Uniprot Accession Number

Q99497

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: NIH3T3 cell lysate ; ; Lane 4.: Mouse brain tissue lysate ; ; Lane 5.: Mouse liver tissue lysate The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Kidney tissue lysate {ATGA0292-WB2.jpg}

FACS Description

Flow cytometry analysis of PARK7 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of PARK7 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0292-FC2.jpg}

IF Description

ICC/IF analysis of PARK7 in Hep3B cells. The cell was stained with ATGA0292 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of PARK7 in HeLa cells. The cell was stained with ATGA0292 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0292-IF2.jpg}