Human STOM antibody

STOM (stomatin) is a member of a highly conserved family of integral membrane proteins. This protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of STOM is associated with hereditary stomatocytosis, a form of hemolytic anemia. Although the wide distribution of stomatin and its constitutive expression suggest an important role for this protein in cell biology, perhaps as a house-keeping component, its function remains undetermined. The massive presence of stomatin in membrane-protruding folds and extensions suggests a possible structural role for this protein in the formation of these structures and/or the anchorage to the actin cytoskeleton.

Product Specifications

Product Name Alternative

Erythrocyte band 7 integral membrane protein isoform a, BND7, EPB7, EPB72

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human STOM (55-288aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT33F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

STOM, ATGA0291-10 µg, ATGA0291-20 µg, ATGA0291-50 µg, ATGA0291-100 µg, ATGA0291-250 µg, ATGA0291-500 µg, ATGA0291-1 mg, ATGA0291-10, ATGA0291-20, ATGA0291-50, ATGA0291-100, ATGA0291-250, ATGA0291-500, ATGA0291-1

References & Citations

Hiebl-Dirschmied CM., et al. (1991) Biochim Biophys Acta 1090 (1) : 123-4.; ; Stewart GW (1997) J. Biol. Chem. 29 (2) : 271-4.; ; Entrez Gene: STOM stomatin.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_004090

Uniprot Accession Number

P27105

WB Description

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate; ; Lane 2.: STOM Transfected 293T cell lysate {ATGA0291-WB2.jpg}

IF Description

ICC/IF analysis of STOM in HeLa cells. The cell was stained with ATGA0291 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .