Human ING2 antibody

ING2 is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. This protein seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Also, It is component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides.

Product Specifications

Product Name Alternative

Inhibitor of growth protein 2, ING1L, P33ing2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ING2 (1-280aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1b κ

Clone

AT39E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ING2, ATGA0290-10 µg, ATGA0290-20 µg, ATGA0290-50 µg, ATGA0290-100 µg, ATGA0290-250 µg, ATGA0290-500 µg, ATGA0290-1 mg, ATGA0290-10, ATGA0290-20, ATGA0290-50, ATGA0290-100, ATGA0290-250, ATGA0290-500, ATGA0290-1

References & Citations

Entrez Gene: ING2 inhibitor of growth family, member 2.; ; Shimada Y., et al. (1999) Cytogenet Cell Genet 83 (3-4) : 232-5.; ; Nagashima M., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98: 9671-9676.; ; Gozani O., et al. (2003) Cell 114: 99-111.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001555

Uniprot Accession Number

Q9H160

WB Description

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ING2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of ING2 in Hep3B cells. The cell was stained with ATGA0290 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .