Human PCBP1 antibody

PCBP-1 and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. PCBP1 constitutes a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA.

Product Specifications

Product Name Alternative

Poly (rC) binding protein 1, Poly (rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PCBP1 (1-163aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2A10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PCBP1, ATGA0284-10 µg, ATGA0284-20 µg, ATGA0284-50 µg, ATGA0284-100 µg, ATGA0284-250 µg, ATGA0284-500 µg, ATGA0284-1 mg, ATGA0284-10, ATGA0284-20, ATGA0284-50, ATGA0284-100, ATGA0284-250, ATGA0284-500, ATGA0284-1

References & Citations

Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24 (10) :1285-1292.; ; Leffers H., et al. (1995) Eur J Biochem 230 (2) :447-53.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006187

Uniprot Accession Number

Q15365

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: 293T cell lysate ; ; Lane 5.: Jurkat cell lysate ; ; Lane 6.: MCF7 cell lysate

IF Description

ICC/IF analysis of PCBP1 in HeLa cells. The cell was stained with ATGA0284 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PCBP1 in MCF-7 cells. The cell was stained with ATGA0284 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0284-IF2.jpg}