Human SORD antibody

SORD, also known as sorbitol dehydrogenase, is a 357 amino acid protein of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney, lens of the eye and malignant prostate tissue. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. SORD deficiency may be associated with diabetic complications such as cataracts and microvascular problems.

Product Specifications

Product Name Alternative

Sorbitol dehydrogenase, Sorbitol dehydrogenase L iditol 2 dehydrogenase, SDH, SORD, SORD 1, SORD1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SORD (1-357aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT10F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SORD, ATGA0283-10 µg, ATGA0283-20 µg, ATGA0283-50 µg, ATGA0283-100 µg, ATGA0283-250 µg, ATGA0283-500 µg, ATGA0283-1 mg, ATGA0283-10, ATGA0283-20, ATGA0283-50, ATGA0283-100, ATGA0283-250, ATGA0283-500, ATGA0283-1

References & Citations

Frenette. G., et al. (2006) J Androl 27: 233-239. ; ; Szabo. Z., et al. (2010) Oncol Rep 23: 1233-1239.; ; Lanaspa. M.A., et al. (2009) J Biol Chem 284: 19974-19981.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

AAH25295

Uniprot Accession Number

Q00796

WB Description

The recombinant protein (20ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SORD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SORD recombinant protein ; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: HeLa cell lysate;

IF Description

ICC/IF analysis of SORD in Hep3B cells. The cell was stained with ATGA0283 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .