Human BMP-2 antibody

BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factors that belong to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.

Product Specifications

Product Name Alternative

Bone morphogenetic protein 2, BMP-2A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BMP2 (283-396aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT15B3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD159 antigen-like family member A, Bone Morphogenetic protein-2, Bone morphogenetic Protein 2, BMP-2A, BMP2A, BMP2 (monomer), BMP2, ATGA0281-10 µg, ATGA0281-20 µg, ATGA0281-50 µg, ATGA0281-100 µg, ATGA0281-250 µg, ATGA0281-500 µg, ATGA0281-1 mg, ATGA0281-010, ATGA0281-020, ATGA0281-050, ATGA0281-100, ATGA0281-250, ATGA0281-500, ATGA0281-01M

References & Citations

Chen D., et al. (2004) . Growth factors. 22 (4) :233-41. ; ; Schliephake H., et al. (2005) . Clin Oral Implants Res. 17 (6) :666-72.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001191

Uniprot Accession Number

P12643

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate