Human PPM1F antibody

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Product Specifications

Product Name Alternative

Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPM1F (1-454aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6D11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PPM1F, ATGA0278-10 µg, ATGA0278-20 µg, ATGA0278-50 µg, ATGA0278-100 µg, ATGA0278-250 µg, ATGA0278-500 µg, ATGA0278-1 mg, ATGA0278-10, ATGA0278-20, ATGA0278-50, ATGA0278-100, ATGA0278-250, ATGA0278-500, ATGA0278-1

References & Citations

Koh, CG., et al. (2002) Curr Biol 12 (4) : 317-21.; ; Tan, KM., et al. (2001) J Biol Chem 276 (47) : 44193-202.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_055449

Uniprot Accession Number

P49593

WB Description

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: PPM1F Transfected 293T cell lysate {ATGA0278-WB2.jpg}

IF Description

ICC/IF analysis of PPM1F in HeLa cells. The cell was stained with ATGA0278 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .