Human SSR2 antibody

SSR2 belongs to the TRAP-beta family. SSR is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. The SSR consists of 2 subunits, a 34kD glycoprotein (alpha-SSR or SSR1) and a 22kD glycoprotein (beta-SSR or SSR2) .

Product Specifications

Product Name Alternative

Tanslocon-associated protein subunit beta precursor, Tanslocon-associated protein subunit beta precursor, HSD25, TLAP, TRAP-BETA, TRAPB

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SSR2 (18-149aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT31G6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SSR2, ATGA0277-10 µg, ATGA0277-20 µg, ATGA0277-50 µg, ATGA0277-100 µg, ATGA0277-250 µg, ATGA0277-500 µg, ATGA0277-1 mg, ATGA0277-10, ATGA0277-20, ATGA0277-50, ATGA0277-100, ATGA0277-250, ATGA0277-500, ATGA0277-1

References & Citations

Chinen, K., et al. (1995) Cytogenet Cell Genet 70 (3-4) : 215-7.; ; Sikorska, M., et al. (2008) J Neurosci Res 86 (8) : 1680-93.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003136

Uniprot Accession Number

P43308

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate

IF Description

ICC/IF analysis of SSR2 in HeLa cells. The cell was stained with ATGA0277 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .