Human Enolase 2/ENO2 antibody

Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.

Enolase2, Neuron-specific Enolase, Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase., ATGA0273-10 µg, ATGA0273-20 µg, ATGA0273-50 µg, ATGA0273-100 µg, ATGA0273-250 µg, ATGA0273-500 µg, ATGA0273-1 mg, ATGA0273-10, ATGA0273-20, ATGA0273-50, ATGA0273-100, ATGA0273-250, ATGA0273-500, ATGA0273-1

Oliva D, Cali L, Feo S, Giallongo A (1991) . Genomics 10 (1) : 157-65.; ; Craig SP, Day IN, Thompson RJ, Craig IW (1991) . Cytogenet. Cell Genet. 54 (1-2) : 71-3.; ; McAleese SM, Dunbar B, Fothergill JE, et al. (1989) . Eur. J. Biochem. 178 (2) : 413-7.;

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: NSE Transfected 293T cell lysate {ATGA0273-WB2.jpg} The Cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: U-87 MG cell lysate ; ; Lane 2.: Jurkat cell lysate {ATGA0273-WB3.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant protein ENO1 ; ; Lane 2.: Recombinant protein NSE (ENO2) ; ; Lane 3.: Recombinant protein ENO3 {ATGA0273-WB4.jpg}

ICC/IF analysis of NSE in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NSE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .