Human CDK4 antibody

Cyclin-dependent kinase 4, also known as CDK4, belongs to the cyclin-dependent kinases (CDK) family. CDKs are a family of protein kinases first discovered for their role in regulating the cell cycle. They are also involved in regulating transcription, mRNA processing, and the differentiation of nerve cells. CDK4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin up-regulates CDK4 activity, whereas binding to the CDK inhibitor p16 down-regulates CDK4 activity.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase 4, CMM3, PSK-J3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CDK4 (1-303aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT6D10

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CDK4, ATGA0272-10 µg, ATGA0272-20 µg, ATGA0272-50 µg, ATGA0272-100 µg, ATGA0272-250 µg, ATGA0272-500 µg, ATGA0272-1 mg, ATGA0272-10, ATGA0272-20, ATGA0272-50, ATGA0272-100, ATGA0272-250, ATGA0272-500, ATGA0272-1

References & Citations

Coleman KG, et al. (1997) . J. Biol. Chem. 272 (30) : 18869-74.; ; Li J, Melvin WS, Tsai MD, Muscarella P (2004) . Biochemistry 43 (14) : 4394-9.; ; Dai K, Kobayashi R, Beach D (1996) . J. Biol. Chem. 271 (36) : 22030-4.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_000066

Uniprot Accession Number

P11802

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;