Human PARP2 antibody

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Product Specifications

Product Name Alternative

Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PARP2 (233-583aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT29G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PARP2, ATGA0270-10 µg, ATGA0270-20 µg, ATGA0270-50 µg, ATGA0270-100 µg, ATGA0270-250 µg, ATGA0270-500 µg, ATGA0270-1 mg, ATGA0270-10, ATGA0270-20, ATGA0270-50, ATGA0270-100, ATGA0270-250, ATGA0270-500, ATGA0270-1

References & Citations

Ame JC, Rolli V, Schreiber V et al. (1999) . J. Biol. Chem. 274 (25) : 17860-8.; ; Schreiber V, Ame JC, Dolle P et al. (2002) . J. Biol. Chem. 277 (25) : 23028-36.; ; Maeda Y, Hunter TC, Loudy DE et al. (2006) . J. Biol. Chem. 281 (14) : 9600-6.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005475

Uniprot Accession Number

Q9UGN5

WB Description

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Raji cell lysate; ; Lane 2. : NIH-3T3 cell lysate; ; {ATGA0270-WB2.jpg}

FACS Description

Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0270 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .

IF Description

ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0270 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .