Human REXO2 antibody

REX2, RNA exonuclease 2 homolog (S. cerevisiae), also known as REXO2, is an enzyme which in humans is encoded by the REXO2 gene. Nucleases are components of DNA and RNA metabolism that carry out functions in DNA repair that also may be involved in resistance of human cells to UV-C-induced cell death through its role in the DNA repair process, replication, and recombination and in RNA processing and degradation. SFN is a homolog of Orn, a 3-prime-to-5-prime exoribonuclease of E. coli that attacks the free 3-prime hydroxyl group on single-stranded RNA, releasing 5-prime mono-nucleotides in a sequential manner.

Product Specifications

Product Name Alternative

Oligoribonuclease mitochondrial, Oligoribonuclease, mitochondrial, REX2, RNA exonuclease 2 homolog (S. cerevisiae), CGI-114, REX2, RFN, SFN

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human REXO 2 (26-237aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT59D9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

REXO2, Oligoribonuclease mitochondrial, Oligoribonuclease, mitochondrial, REX2, RNA exonuclease 2 homolog (S. cerevisiae), CGI-114, REX2, RFN, SFN, ATGA0269-10 µg, ATGA0269-20 µg, ATGA0269-50 µg, ATGA0269-100 µg, ATGA0269-250 µg, ATGA0269-500 µg, ATGA0269-1 mg, ATGA0269-10, ATGA0269-20, ATGA0269-50, ATGA0269-100, ATGA0269-250, ATGA0269-500, ATGA0269-1

References & Citations

Entrez Gene: REXO2 REX2, RNA exonuclease 2 homolog (S. cerevisiae) ; ; Lai, CH., et al. (2000) Genome Res 10 (5) : 703-13; ; Nguyen, LH., et al. (2000) J.Biol.Chem 275 (34) : 25900-6

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_056338

Uniprot Accession Number

Q9Y3B8

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human REXO2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : SW480 cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : Jurkat cell lyate ; ; Lane 5. : K562 cell lysate ; ; Lane 6. : NIH3T3 cell lysate

IF Description

ICC/IF analysis of REXO2 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human REXO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0269-IF1b.jpg} ICC/IF analysis of REXO2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human REXO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0269-IF2a.jpg} {ATGA0269-IF2b.jpg}