Human NBL1 antibody

NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development and alternatively spliced transcript variants have been identified for this gene. Also, as a tumor suppressor gene of neuroblastoma may play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 have been observed.

Product Specifications

Product Name Alternative

Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NBL1 (18-181aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT38G8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DAN, NBL1, ATGA0267-10 µg, ATGA0267-20 µg, ATGA0267-50 µg, ATGA0267-100 µg, ATGA0267-250 µg, ATGA0267-500 µg, ATGA0267-1 mg, ATGA0267-10, ATGA0267-20, ATGA0267-50, ATGA0267-100, ATGA0267-250, ATGA0267-500, ATGA0267-1

References & Citations

Olakowski, M., et al. (2009) Folia Histochem Cytobiol 47 (2) : 249-55.; ; Shaikhibrahim, Z., et al. (2011) Int J Mol Med 28 (4) : 605-11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_005371

Uniprot Accession Number

P41271

WB Description

The cell lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate;

FACS Description

Flow cytometry analysis of NBL1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of NBL1 in Jurkat cells. The cell was stained with ATGA0267 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of NBL1 in A549 cells. The cell was stained with ATGA0267 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010222533296.jpg}