Human Cathepsin S antibody

CTSS (Cathepsin S) is a lysosomal enzyme that belongs to the papain family of cysteine proteases. This protein is expressed by antigen presenting cells including macrophages, B-lymphocytes, dendritic cells and microglia. Moreover, CTSS is expressed in some epithelial cells. In contrast, cortical thymic epithelial cells do not express CTSS. Normal epidermal keratinocytes do not express CTSS. However, level of CTSS is elevated in psoriatic keratinocytes due to stimulation by proinflammatory factors. CTSS can function as an elastase over a broad pH range in alveolar macrophages.

Product Specifications

Product Name Alternative

Cathepsin S, MGC3886

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTSS (17-331aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1F9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cathepsin S, CTSS, ATGA0264-10 µg, ATGA0264-20 µg, ATGA0264-50 µg, ATGA0264-100 µg, ATGA0264-250 µg, ATGA0264-500 µg, ATGA0264-1 mg, ATGA0264-10, ATGA0264-20, ATGA0264-50, ATGA0264-100, ATGA0264-250, ATGA0264-500, ATGA0264-1

References & Citations

Shi. G.P., et al. (1994) J Biol Chem 269: 11530-11536.; ; Lemere. C.A., et al. (1995) Am J Pathol 146 (4) : 848-860.; ; Shi. G.P., et al. (1992) J Biol Chem 267 (11) : 7258-7262.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_004070

Uniprot Accession Number

P25774

WB Description

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human CTSB ; ; Lane 2.: Recombinant Human CTSD ; ; Lane 3.: Recombinant Human CTSE ; ; Lane 4.: Recombinant Human CTSF ; ; Lane 5.: Recombinant Human CTSH ; ; Lane 6.: Recombinant Human CTSK ; ; Lane 7.: Recombinant Human CTSL ; ; Lane 8.: Recombinant Human CTSS ; ; Lane 9.: Recombinant Human CTSW ; ; Lane 10.: Recombinant Human CTSZ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: U87MG cell lysate; ; Lane 3.: A549 cell lysate {ATGA0264-WB2.jpg}

IF Description

ICC/IF analysis of CTSS in U87MG cells. The cell was stained with ATGA0264 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of CTSS in HeLa cells. The cell was stained with ATGA0264 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0264-IF2.jpg}ICC/IF analysis of CTSS in A549 cells. The cell was stained with ATGA0264 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0264-IF3.jpg}