Human ICT1 antibody

Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.

Product Specifications

Product Name Alternative

Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ICT1 (30-206aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E9

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ICT1, ATGA0263-10 µg, ATGA0263-20 µg, ATGA0263-50 µg, ATGA0263-100 µg, ATGA0263-250 µg, ATGA0263-500 µg, ATGA0263-1 mg, ATGA0263-10, ATGA0263-20, ATGA0263-50, ATGA0263-100, ATGA0263-250, ATGA0263-500, ATGA0263-1

References & Citations

Richter R., et al. (2010) EMBO J. 29:1116-1125.; ; van Belzen N., et al. (1995) Eur. J. Biochem. 234:843-848.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001536

Uniprot Accession Number

Q14197

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: ICT Transfected 293T cell lysate {ATGA0263-WB2.jpg}

FACS Description

Flow cytometry analysis of ICT1 in A549 cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.