Human CNBP antibody

CNBP is a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. It functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2.

Product Specifications

Product Name Alternative

Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CNBP (1-170aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT38F10

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CNBP, Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9, ATGA0262-10 µg, ATGA0262-20 µg, ATGA0262-50 µg, ATGA0262-100 µg, ATGA0262-250 µg, ATGA0262-500 µg, ATGA0262-1 mg, ATGA0262-10, ATGA0262-20, ATGA0262-50, ATGA0262-100, ATGA0262-250, ATGA0262-500, ATGA0262-1

References & Citations

McGrath CF, Buckman JS, et al. (2003) . J Virol. 77 (15) :8524-31.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1000. Recommended starting dilution is 1:1000.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001120668

Uniprot Accession Number

P62633

WB Description

The cell lysate of 293T (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNBP antibody (1:250 - 1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of CNBP in MCF7 cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CNBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0262-IF1b.jpg} ICC/IF analysis of CNBP in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0262-IF2a.jpg} {ATGA0262-IF2b.jpg}