What is the catalog number for Human CIAO1 antibody?

The catalog number for Human CIAO1 antibody is ATGA0261-100.

What sizes are available for Human CIAO1 antibody?

Human CIAO1 antibody is available in the following size(s): 100 µL.

Is Human CIAO1 antibody in stock?

Human CIAO1 antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human CIAO1 antibody

CIAO1 is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. As part of the mitotic spindle-associated MMXD complex, it may play a role in chromosome segregation.

Product Specifications

Product Name Alternative

Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CIAO1 (1-339aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6C9

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CIAO1, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39, ATGA0261-10 µg, ATGA0261-20 µg, ATGA0261-50 µg, ATGA0261-100 µg, ATGA0261-250 µg, ATGA0261-500 µg, ATGA0261-1 mg, ATGA0261-10, ATGA0261-20, ATGA0261-50, ATGA0261-100, ATGA0261-250, ATGA0261-500, ATGA0261-1

References & Citations

Johnstone, RW., et al. (1998) J Biol Chem 273 (18) : 10880-7.; ; Srinivasan, V., et al. (2007) Structure 15: 1246-1257.; ; Ito, S., et al. (2010) Mol Cell 39: 632-640.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004795

Uniprot Accession Number

O76071

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIAO1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIAO1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: CIAO1 transfected 293T cell lysate {2004010217332740.jpg}