Human MRPS25 antibody

MRPS25 is a 28S subunit protein that belongs to the ribosomal protein S5P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. A pseudogene corresponding to this gene is found on chromosome 4

Product Specifications

Product Name Alternative

28S ribosomal protein S25 mitochondrial, 28S ribosomal protein S25, mitochondrial, mitochondrial ribosomal protein S25, MRP-S25, RPMS25

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MRPS25 (1-173aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT38E7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MRPS25, 28S ribosomal protein S25 mitochondrial, 28S ribosomal protein S25, mitochondrial, mitochondrial ribosomal protein S25, MRP-S25, RPMS25, ATGA0258-10 µg, ATGA0258-20 µg, ATGA0258-50 µg, ATGA0258-100 µg, ATGA0258-250 µg, ATGA0258-500 µg, ATGA0258-1 mg, ATGA0258-10, ATGA0258-20, ATGA0258-50, ATGA0258-100, ATGA0258-250, ATGA0258-500, ATGA0258-1

References & Citations

Koc EC, Burkhart W, Blackburn K, et al. (2000) . J. Biol. Chem. 275 (42) : 32585-91.; ; Zhang Z, Gerstein M (2003) . Genomics 81 (5) : 468-80.; ; Cavdar Koc E, et al. (May 2001) . J Biol Chem 276 (22) : 19363-74.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_071942

Uniprot Accession Number

P82663

WB Description

The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: anti-human MRPS25 antibody 1:500; ; Lane 2.: anti-human MRPS25 antibody 1:1000; ; Lane 3.: anti-human MRPS25 antibody 1:5000