Human Cytokeratin 19 antibody

KRT19 (type 1 cytokeratin 19) is 44kDa intermediate filament (IF) protein that is part of the Keratin family of proteins. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Product Specifications

Product Name Alternative

Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, CK19, K19, K1CS

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT19 (1-400aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT13D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cytokeratin19, ATGA0255-10 µg, ATGA0255-20 µg, ATGA0255-50 µg, ATGA0255-100 µg, ATGA0255-250 µg, ATGA0255-500 µg, ATGA0255-1 mg, ATGA0255-10, ATGA0255-20, ATGA0255-50, ATGA0255-100, ATGA0255-250, ATGA0255-500, ATGA0255-1

References & Citations

Stone, M.R., et al. (2005) Mol Biol Cell 16: 4280-4293.; ; Lacroix, M. (2006) Endocrine Related Cancer 13 (4) : 1033-1067.; ; Allard W.J., et al. (2004) Clin Cancer Research 10 (20) : 6897-6904.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_002267

Uniprot Accession Number

P08727

WB Description

The cell lysate of MCF7 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate {ATGA0255-WB2.jpg} The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: KRT19 Transfected 293T cell lysate {ATGA0255-WB3.jpg}

FACS Description

Flow cytometry analysis of KRT19 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of KRT19 in HepG2 cells. The cell was stained with ATGA0255 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .