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Human SNTA1 antibody

SNTA1 (Syntrophin alpha 1) is 505 amino acid protein. SNTA1 is adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. It may link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. SNTA1 plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Defects of SNTA1 are the cause of long QT syndrome type 12 (LQT12) .

Product Specifications

Product Name Alternative

Syntrophin alpha 1, Syntrophin, alpha 1, dJ1187J4.5, LQT12, SNT1, TACIP1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SNTA1 (1-505aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1E1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SNTA1, ATGA0254-10 µg, ATGA0254-20 µg, ATGA0254-50 µg, ATGA0254-100 µg, ATGA0254-250 µg, ATGA0254-500 µg, ATGA0254-1 mg, ATGA0254-10, ATGA0254-20, ATGA0254-50, ATGA0254-100, ATGA0254-250, ATGA0254-500, ATGA0254-1

References & Citations

Wu, G., et al. (2008) Circ Arrhythm Electrophysiol 1: 193-201.; ; Ahn, A.H., et al. (1996) J Biol Chem 271 (5) : 2724-30.; ; Yang, B., et al (1995) J Biol Chem 270 (10) : 4975-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_003089

Uniprot Accession Number

Q13424

WB Description

The cell lysate of HepG2 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNTA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of SNTA1 in HeLa cells. The cell was stained with ATGA0254 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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