Human Keratin 8/KRT8 antibody

Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Product Specifications

Product Name Alternative

KO, Keratin type II cytoskeletal 8, Keratin 8, K8, K2C8, CYK8, CK8, CARD2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT8 (1-483aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

AT3D11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Type II cytoskeletal 8, KO, Keratin8, Keratin type II cytoskeletal 8, Keratin 8, Keratin, K-8, K8, K2C-8, K2C8, K2C 8, K 8, Cytokeratin8, Cytokeratin 8, CYK8, CYK 8, CK-8, CK8, CK 8, CARD-2, CARD2, CARD 2, ATGA0253-10 µg, ATGA0253-20 µg, ATGA0253-50 µg, ATGA0253-100 µg, ATGA0253-250 µg, ATGA0253-500 µg, ATGA0253-1 mg, ATGA0253-010, ATGA0253-020, ATGA0253-050, ATGA0253-100, ATGA0253-250, ATGA0253-500, ATGA0253-01M

References & Citations

Moriya, T ., et al. (2006) Med Mol Morphol 39 (1) : 8-13; ; Leitner, A., et al. (2012) Biophys J 103 (2) : 195-201

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_002264

Uniprot Accession Number

P05787

WB Description

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: LnCap cell lysate ; ; Lane 2.: PC3 cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: A549 cell lysate {ATGA0253-WB2.jpg}

FACS Description

Flow cytometry analysis of KRT8 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of KRT8 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0253-IF1b.jpg}ICC/IF analysis of KRT8 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0253-IF2a.jpg}{ATGA0253-IF2b.jpg}