Human TDG antibody

G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Product Specifications

Product Name Alternative

Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TDG (1-410aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TDG, Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase, ATGA0251-10 µg, ATGA0251-20 µg, ATGA0251-50 µg, ATGA0251-100 µg, ATGA0251-250 µg, ATGA0251-500 µg, ATGA0251-1 mg, ATGA0251-10, ATGA0251-20, ATGA0251-50, ATGA0251-100, ATGA0251-250, ATGA0251-500, ATGA0251-1

References & Citations

Neddermann, P ., et al. (1996) J Biol Chem 271 (22) : 12767-74; ; Sard, L., et al. (1997) Genomics 44 (2) : 222-6; ; Gallinari, P., et al. (1996) Nature 383 (6602) : 735-8

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH37557

Uniprot Accession Number

Q13569

WB Description

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of TDG in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TDG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0251-IF1b.jpg}