Human ADI1 antibody

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14.

Product Specifications

Product Name Alternative

Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ADI1 (1-179aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT27E8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ADI1, ATGA0248-10 µg, ATGA0248-20 µg, ATGA0248-50 µg, ATGA0248-100 µg, ATGA0248-250 µg, ATGA0248-500 µg, ATGA0248-1 mg, ATGA0248-10, ATGA0248-20, ATGA0248-50, ATGA0248-100, ATGA0248-250, ATGA0248-500, ATGA0248-1

References & Citations

Gotoh I, Uekita T, Seiki M (2007) . Genes Cells 12 (1) : 105-17.; ; Oram SW, Ai J, Pagani GM, et al. (2007) . Neoplasia 9 (8) : 643-51.; ; Yeh CT, Lai HY, Chen TC, et al. (2001) . J. Virol. 75 (22) : 11017-24.

Other References

Bae DH, et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. (Biochim Biophys Acta Mol Basis Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/32480040/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_060739.1

Uniprot Accession Number

Q9BV57

WB Description

The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADI1 antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;

IF Description

ICC/IF analysis of ADI1 in Hep3B cells. The cell was stained with ATGA0248 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .