Human MyD88 antibody

Myeloid differentiation primary response gene 88 (MYD88) is adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein increases IL-8 transcription. It is involved in IL-18-mediated signaling pathway. MYD88 activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes.

Product Specifications

Product Name Alternative

Myeloid differentiation primary response protein MyD88, MYD88D

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MYD88 (1-309aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT22F11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MyD88, ATGA0247-10 µg, ATGA0247-20 µg, ATGA0247-50 µg, ATGA0247-100 µg, ATGA0247-250 µg, ATGA0247-500 µg, ATGA0247-1 mg, ATGA0247-10, ATGA0247-20, ATGA0247-50, ATGA0247-100, ATGA0247-250, ATGA0247-500, ATGA0247-1

References & Citations

Bonnert TP, et al. (January 1997) . FEBS Letters 402 (1) : 81-4.; ; Lord KA, et al. (1990) . Oncogene 5 (7) : 1095-7.; ; Arancibia SA, et al. (2007) . Biological research 40 (2) : 97-112.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002459

Uniprot Accession Number

Q99836

WB Description

The cell lysate of Raji (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYD88 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of MYD88 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of MYD88 in A549 cells. The cell was stained with ATGA0247 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .