Human ACOT7 antibody

ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.

Product Specifications

Product Name Alternative

Acyl-CoA thioesterase 7, ACH1, ACT, BACH, CTE-II, hBACH, LACH, LACH1, MGC1126, RP1-120G22

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACOT7 (1-370aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACOT7, ATGA0245-10 µg, ATGA0245-20 µg, ATGA0245-50 µg, ATGA0245-100 µg, ATGA0245-250 µg, ATGA0245-500 µg, ATGA0245-1 mg, ATGA0245-10, ATGA0245-20, ATGA0245-50, ATGA0245-100, ATGA0245-250, ATGA0245-500, ATGA0245-1

References & Citations

Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_009205

Uniprot Accession Number

O00154

WB Description

The 293T cell lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of ACOT7 in 293T cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ACOT7 in U87MG cells. The cell was stained with ATGA0245 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .