Human PDZK1 antibody

PDZK1 is PDZ domain-containing scaffolding protein with a 519 amino acid protein. PDZK1 mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. This protein is expressed in the kidney, pancreas, liver, gastrointestinal tract, and adrenal cortex. In situ hybridization experiments showed that the expression of PDZK1 was limited to epithelial cells. In addition, PDZK1 was overexpressed in selected tumors of epithelial origin.

Product Specifications

Product Name Alternative

Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDZK1 (1-519aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PDZK1, ATGA0243-10 µg, ATGA0243-20 µg, ATGA0243-50 µg, ATGA0243-100 µg, ATGA0243-250 µg, ATGA0243-500 µg, ATGA0243-1 mg, ATGA0243-10, ATGA0243-20, ATGA0243-50, ATGA0243-100, ATGA0243-250, ATGA0243-500, ATGA0243-1

References & Citations

Kocher, O., et al. (1999) Lab Invest 64: 1733-1745.; ; Kocher, O., et al. (2001) Mol Cell Biol 23: 1175-1180.; ; Gisler, S.M., et al (2003) Kidney Int 64 (5) : 1733-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001188254

Uniprot Accession Number

Q5T2W1

WB Description

The cell lysates of HeLa (40ug) and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDZK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of PDZK1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of PDKZ1 in Hep3B cells. The cell was stained with ATGA0243 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .