Human CISD1 antibody

CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.

Product Specifications

Product Name Alternative

DGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CISD1 (32-108aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1A8

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CISD1, ATGA0242-10 µg, ATGA0242-20 µg, ATGA0242-50 µg, ATGA0242-100 µg, ATGA0242-250 µg, ATGA0242-500 µg, ATGA0242-1 mg, ATGA0242-10, ATGA0242-20, ATGA0242-50, ATGA0242-100, ATGA0242-250, ATGA0242-500, ATGA0242-1

References & Citations

Wiley, S.E., et al. (2007) J Biol Chem 282: 23745-23749.; ; Paddock, M.L., et al. (2007) Proc Natl Acad Sci USA 104: 14342-14347.; ; Taminelli, G.L., et al. (2008) Biochem Biophys Res Commun 365: 856-862.

Other References

He QQ, et al. MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27748416/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_060934

Uniprot Accession Number

Q9NZ45

WB Description

The tissue lysate of Mouse liver (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: MCF7 cell lysate {ATGA0242-WB2.jpg}

FACS Description

Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .