Human SAR1B antibody

SAR1B (GTP-binding protein SAR1b) is 198 amino acid protein. SAR1B is involved in transport from the endoplasmic reticulum to the Golgi apparatus. This protein is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Mutations in the SAR1B gene are associated with CMRD which is autosomal recessive disorder of severe fat malabsorption.

Product Specifications

Product Name Alternative

GTP-binding protein SAR1b, ANDD, CMRD, GTBPB, SARA2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SAR1B (1-198aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1C7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SAR1B, ATGA0241-10 µg, ATGA0241-20 µg, ATGA0241-50 µg, ATGA0241-100 µg, ATGA0241-250 µg, ATGA0241-500 µg, ATGA0241-1 mg, ATGA0241-10, ATGA0241-20, ATGA0241-50, ATGA0241-100, ATGA0241-250, ATGA0241-500, ATGA0241-1

References & Citations

Jones, B., et al. (2003) Nat Genet 34 (1) : 29-31.; ; Annesi, G., et al. (2007) Clin Genet 71 (3) : 288-9.; ; Treepongkaruna, S., et al. (2009) J Pediatr Gastroenterol Nutr 48 (3) : 370-3.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057187

Uniprot Accession Number

Q9Y6B6

WB Description

The tissue lysate of Mouse muscle (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SAR1B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;

FACS Description

Flow cytometry analysis of SAR1B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of SAR1B in Hep3B cells. The cell was stained with ATGA0241 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .