Human ACAT1 antibody

ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.

Product Specifications

Product Name Alternative

Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACAT1 (34-427aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2C5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACAT1, ATGA0240-10 µg, ATGA0240-20 µg, ATGA0240-50 µg, ATGA0240-100 µg, ATGA0240-250 µg, ATGA0240-500 µg, ATGA0240-1 mg, ATGA0240-10, ATGA0240-20, ATGA0240-50, ATGA0240-100, ATGA0240-250, ATGA0240-500, ATGA0240-1

References & Citations

Antonenkov, V.D., et al. (2000) Eur J Biochem 267: 2981-2990.; ; Korman, S.H. (2006) Mol Genet Metab 89: 289-299.; ; Yamaguchi, S., et al. (1988) J Clin Invest 81 (3) : 813-817.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_000010

Uniprot Accession Number

P24752

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Hep3B cell lysate; ; Lane 2. : 293T cell lysate; ; Lane 3. : A431 cell lysate {ATGA0240-WB2.jpg}

IF Description

ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained with ATGA0240 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .