Human MRRF antibody

Mitochondrial ribosome recycling factor, also known as MRRF, is a 262 amino acid protein that belongs to the ribosome recycling factor (RRF) family. Localized to mitochondria, MRRF is responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. It may increase the efficiency of translation by recycling ribosomes from one round of translation to another. MRRF belongs to the RRF family. 8 isoforms of the human protein are produced by alternative splicing.

Product Specifications

Product Name Alternative

Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MRRF (56-262aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7D10

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MRRF, ATGA0237-10 µg, ATGA0237-20 µg, ATGA0237-50 µg, ATGA0237-100 µg, ATGA0237-250 µg, ATGA0237-500 µg, ATGA0237-1 mg, ATGA0237-10, ATGA0237-20, ATGA0237-50, ATGA0237-100, ATGA0237-250, ATGA0237-500, ATGA0237-1

References & Citations

Checcarelli, N. et al. (May 1994) . J Neurol Sci. 123 (1-2) :74-9.; ; Janosi, L. et al. (1996) . Biochimie. 78 (11-12) :959-69.; ; Zavialov, A.V. et al. (Jun 2005) . Mol Cell. 1018 (6) :675-86.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_620132

Uniprot Accession Number

Q96E11

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate