Human CNDP2/CPGL antibody

CNDP2, known as tissue carnosinase and peptidase A, is a nonspecific dipeptidase rather than a selective carnosinase and is a secreted peptidase homologous to M20 peptidases. Also known as glutamate carboxypeptidase-like protein 1 (CPGL), CNDP2 is ubiquitously expressed throughout human tissue. CNDP2 Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly and may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells.

Product Specifications

Product Name Alternative

Cytosolic non-specific dipeptidase, CN2, CPGL, HsT2298, PEPA

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CNDP2 (1-475aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT15E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CNDP2, CPGL, ATGA0236-10 µg, ATGA0236-20 µg, ATGA0236-50 µg, ATGA0236-100 µg, ATGA0236-250 µg, ATGA0236-500 µg, ATGA0236-1 mg, ATGA0236-10, ATGA0236-20, ATGA0236-50, ATGA0236-100, ATGA0236-250, ATGA0236-500, ATGA0236-1

References & Citations

Zhang, P., et al. (2006) Cancer res 12: 6617-6625; ; Kaur, H., et al. (2009) J.Biol.Chem 284: 14493-14502; ; Licker, V., et al. (2012) J proteomics 375 (15) : 4656-67

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases, Ubiquitination

NCBI Accession Number

NP_060705

Uniprot Accession Number

Q96KP4

WB Description

The tissue lysate of mouse kidney (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNDP2/CPGL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNDP2/CPGL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse liver tissue lysate; ; Lane 2.: HepG2 cell lysate; ; {ATGA0236-WB2.jpg}

IF Description

ICC/IF analysis of CNDP2/CPGL in HeLa cells. The cell was stained with ATGA0236 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .