Human FBP2 antibody

FBP2 (Fructose-1, 6-bisphosphatase isozyme 2) is a 339 amino acid protein. FBP2 belongs to the FBPase class 1 family. The hydrolysis of fructose-1, 6-bisphosphate to fructose-6-phosphate is a key reaction of carbohydrate metabolism. The enzyme that catalyzes this reaction appears to be present in all forms of living organisms. FBPase is encoded by two genes, FBP1 and FBP2, which express the liver and muscle isoforms, respectively. FBPase is regulated by AMP inhibition in most species. Inhibition of FBPase by AMP affects the turnover of bound substrate and not its affinity for substrate.

Product Specifications

Product Name Alternative

Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FBP2 (1-339aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E11

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FBP2, ATGA0235-10 µg, ATGA0235-20 µg, ATGA0235-50 µg, ATGA0235-100 µg, ATGA0235-250 µg, ATGA0235-500 µg, ATGA0235-1 mg, ATGA0235-10, ATGA0235-20, ATGA0235-50, ATGA0235-100, ATGA0235-250, ATGA0235-500, ATGA0235-1

References & Citations

Dzugaj, A., et al. (1980) Biochim Biophys Acta 614: 407-412.; ; Marcus, F., et al. (1987) Arch Biol Med Exp 20: 371-378.; ; Matsuura, T., et al. (2002) Mol Genet Metab 76: 207-210.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_003828

Uniprot Accession Number

O00757

WB Description

The cell lysate of mouse muscle (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FBP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.