Human CMBL antibody

CMBL (carboxymethylenebutenolidase homolog) is a 245 amino acid cytosolic protein and cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. That is ubiquitously expressed with high expression in liver, colon, kidney and small intestine, where it acts as a bioactivating enzyme for a prodrug type angiotensin II type 1 receptor antagonist known as olmesartan medoxomil. CMBL is inhibited by p-chloromercuribenzoate (PCMB) .

Product Specifications

Product Name Alternative

Carboxymethylenebutenolidase homolog

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CMBL (1-245aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2B11

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CMBL, ATGA0233-10 µg, ATGA0233-20 µg, ATGA0233-50 µg, ATGA0233-100 µg, ATGA0233-250 µg, ATGA0233-500 µg, ATGA0233-1 mg, ATGA0233-10, ATGA0233-20, ATGA0233-50, ATGA0233-100, ATGA0233-250, ATGA0233-500, ATGA0233-1

References & Citations

Ishizuka T, et al. (Apr 2010) . J Biol Chem. 16285 (16) :11892-902. ; ; Yang X, et al. (Mar 2010) . J Proteome Res. 59 (3) :1402-15.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_620164

Uniprot Accession Number

Q96DG6

WB Description

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CMBL antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.