Human N-Acetyl-D-Glucosamine Kinase/NAGK antibody

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P (II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.

Product Specifications

Product Name Alternative

N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NAGK (1-344aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4D12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NAGK, ATGA0232-10 µg, ATGA0232-20 µg, ATGA0232-50 µg, ATGA0232-100 µg, ATGA0232-250 µg, ATGA0232-500 µg, ATGA0232-1 mg, ATGA0232-10, ATGA0232-20, ATGA0232-50, ATGA0232-100, ATGA0232-250, ATGA0232-500, ATGA0232-1

References & Citations

Weihofen WA, Berger M, Chen H, et al. (2007) . J. Mol. Biol. 364 (3) : 388-99.; ; Weidanz JA, Campbell P, Moore D, et al. (1997) . Br. J. Haematol. 95 (4) : 645-53.; ; Hinderlich S., et al. (Jul 2000) . Eur J Biochem 267 (11) : 3301-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_060037.2

Uniprot Accession Number

Q9UJ70

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane a.: 293T cell lysate.; ; Lane b.: HeLa cell lysate.

IF Description

ICC/IF analysis of NAGK in HeLa cells. The cell was stained with ATGA0232 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .