What is the catalog number for Human MEMO1 antibody?

The catalog number for Human MEMO1 antibody is ATGA0230-100.

What sizes are available for Human MEMO1 antibody?

Human MEMO1 antibody is available in the following size(s): 100 µL.

Is Human MEMO1 antibody in stock?

Human MEMO1 antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human MEMO1 antibody

MEMO1 (Mediator of ErbB2-driven cell motility 1) is a 297 amino acid protein. It is thought to relax extracellular chemotactic signals that are targeted at the microtubule cytoskeleton, thereby controlling cell migration. Additionally, MEMO1 is required for breast carcinoma migration, suggesting an important role in tumorigenesis. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity.

Product Specifications

Product Name Alternative

Protein MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MEMO1 (1-297aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1E9

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MEMO1, ATGA0230-10 µg, ATGA0230-20 µg, ATGA0230-50 µg, ATGA0230-100 µg, ATGA0230-250 µg, ATGA0230-500 µg, ATGA0230-1 mg, ATGA0230-10, ATGA0230-20, ATGA0230-50, ATGA0230-100, ATGA0230-250, ATGA0230-500, ATGA0230-1

References & Citations

Marone R., et al. (2004) Nat Cell Biol 6 (6) :515-522; ; Meira M., et al. (2009) J Cell Sci 122:787-797; ; Zaoui K., et al. (2010) Proc Natl Acad Sci USA 107 (43) :18517-18522

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057039

Uniprot Accession Number

Q9Y316

WB Description

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: U87MG cell lysate; ; Lane 2.: Mouse brain tissue lysate {ATGA0230-WB2.jpg}

FACS Description

Flow cytometry analysis of MEMO1 in U87MG cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.