Human GCLM antibody

GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.

Product Specifications

Product Name Alternative

Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GCLM (1-274aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2D12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GCLM, ATGA0229-10 µg, ATGA0229-20 µg, ATGA0229-50 µg, ATGA0229-100 µg, ATGA0229-250 µg, ATGA0229-500 µg, ATGA0229-1 mg, ATGA0229-10, ATGA0229-20, ATGA0229-50, ATGA0229-100, ATGA0229-250, ATGA0229-500, ATGA0229-1

References & Citations

Dickinson D.A., et al. (2005) Free Radic Biol Med 37 (8) : 1152-1159.; ; Inoue Y., et al. (2003) Int J Oncol 23 (5) : 1333-1339.; ; Robertson N.G., et al. (1995) Genomics 23 (1) : 42-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_002052

Uniprot Accession Number

P48507

WB Description

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of GCLM in HeLa cells. The cell was stained with ATGA0229 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .