Human Kallikrein 3/PSA antibody

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. PSA is now used to indicate the presence of semen in forensic serology. The semen of adult males has PSA levels far in excess of those found in other tissues; therefore, a high level of PSA found in a sample is an indicator that semen may be present. Because PSA is a biomarker that is expressed independently of spermatozoa, it remains useful in identifying semen from vasectomized and azoospermic males.

Product Specifications

Product Name Alternative

KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS, hK3, KLK2A1, Kallikrein related peptidase 3, Prostate-specific antigen isoform 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KLK3 (25-261aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Seminin, Semenogelase, PSA, Prostate-specific antigen isoform 1, Prostate-specific antigen, Prostate specific antigen isoform 1, Prostate specific antigen, P-30 antigen, P30 antigen, KLK3, KLK2A1, Kallikrein-3, Kallikrein3, Kallikrein related peptidase 3, Kallikrein 3, hK3, Gamma-seminoprotein, APS, Gamma seminoprotein, ATGA0228-10 µg, ATGA0228-20 µg, ATGA0228-50 µg, ATGA0228-100 µg, ATGA0228-250 µg, ATGA0228-500 µg, ATGA0228-1 mg, ATGA0228-010, ATGA0228-020, ATGA0228-050, ATGA0228-100, ATGA0228-250, ATGA0228-500, ATGA0228-01M

References & Citations

Balk SP, Ko YJ, Bubley GJ (January 2003) . J. Clin. Oncol. 21 (2) : 383-91.; ; Lilja H (November 2003) . Urology 62 (5 Suppl 1) : 27-33.; ; Rao AR, Motiwala HG, Karim OM (January 2008) . BJU Int. 101 (1) : 5-10.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001639

Uniprot Accession Number

P07288

WB Description

The extracts of PC-3 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLK3 antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of KLK3 in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of KLK3 in LNCap cells. The cell was stained with ATGA0228 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .