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Human PIG3/TP53I3 antibody

TP53I3 participates in the generation of reactive oxygen species (ROS) . TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1, 2-naphtoquinone over 1, 4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro) . TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.

Product Specifications

Product Name Alternative

Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TP53I3 (1-332aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1C9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NADPH quinone reductase PIG3, NADPH:quinone reductase PIG3, p53 induced gene 3 protein, p53-induced gene 3 protein, PIG3, Putative quinone oxidoreductase, Quinone oxidoreductase homolog, Quinone oxidoreductase PIG3, Quinone oxidoreductase PIG3 p53 induced gene 3 protein, TP53I3, Tumor protein p53 inducible protein 3, Tumor protein p53-inducible protein 3, ATGA0227-10 µg, ATGA0227-20 µg, ATGA0227-50 µg, ATGA0227-100 µg, ATGA0227-250 µg, ATGA0227-500 µg, ATGA0227-1 mg, ATGA0227-010, ATGA0227-020, ATGA0227-050, ATGA0227-100, ATGA0227-250, ATGA0227-500, ATGA0227-01M

References & Citations

Porte S, et al. (2009) J Biol Chem.. 284 (25) :17194-205. ; ; Flatt PM. et al. (2000) Cancer Lett. 156 (1) :63-72.; ; J Biol Chem. (2009 Jun 19) 284 (25) :17194-205.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_671713

Uniprot Accession Number

Q53FA7

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : A549 cell lysate ; ; Lane 2. : SW480 cell lysate The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: TP53I3 Transfected 293T cell lysate {ATGA0227-WB2.jpg}

IF Description

ICC/IF analysis of TP53I3 in A549 cells. The cell was stained with ATGA0227 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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