Human KRT23 antibody

KRT23, also known as cytokeratin 23, is a 422 amino acid intermediate filament protein. The human KRT23 gene is located on chromosome 17q21. 2. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of epithelial cells structure. Cytokeratins are described to be differentiation markers in several epithelial cancers.

Product Specifications

Product Name Alternative

CK-23, CK23, Cytokeratin-23, DKFZP434G032, HAIK1, Cytokeratin 23, MGC26158, K23, K1C23, HAIK 1, Histone deacetylase inducible keratin 23, type I cytoskeletal 23

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT23 (271-422aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KRT23, ATGA0225-10 µg, ATGA0225-20 µg, ATGA0225-50 µg, ATGA0225-100 µg, ATGA0225-250 µg, ATGA0225-500 µg, ATGA0225-1 mg, ATGA0225-10, ATGA0225-20, ATGA0225-50, ATGA0225-100, ATGA0225-250, ATGA0225-500, ATGA0225-1

References & Citations

Birkenkamp-Demtroder K., et al. (2007) Mol Oncol 1 (2) : 181-195.; ; Zhang J.S., et al. (2001) Genes Chromosomes Cancer 30: 123-135.; ; Schweizer J., et al. (2006) Cell Biol 174: 169-174.; ; Rogers M.A., et al. (2004) Differentiation 72: 527-540.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_056330

Uniprot Accession Number

Q9C075

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate

FACS Description

Flow cytometry analysis of KRT23 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of KRT23 in Hep3B cells. The cell was stained with ATGA0225 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .