Human GIPC1/2 antibody

GIPC, GAIP interacting protein at the C terminus, is a PDZ domain containing protein that interacts with RGS-GAIP. GIPC was also identified as TIP-2, a protein that interacts with the viral oncoprotein TAX, which transactivates viral and cellular promoters through interactions with various transcription factors. GIPC specifically localizes to clusters of vesicles near the plasma membrane and participates in G protein-coupled signaling pathway involved in regulating clathrin-coated vesicular trafficking. GIPC also associates with membrane bound semaphorin F (M-SemF), which is involved in neuronal axon growth, and it appears to regulate the subcellular distribution of M-SemF in the brain.

Product Specifications

Product Name Alternative

PDZ domain containing family member 2, SEMCAP2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GIPC2 (1-315aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1G10

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Enolase 2, ENO2, P09104, NP_001966.1, PDZ domain containing family member 2, PDZ domain containing family, member 2, SEMCAP2, GIPC, GIPC1, O14908, NP_974199, ATGA0223-10 µg, ATGA0223-20 µg, ATGA0223-50 µg, ATGA0223-100 µg, ATGA0223-250 µg, ATGA0223-500 µg, ATGA0223-1 mg, ATGA0223-010, ATGA0223-020, ATGA0223-050, ATGA0223-100, ATGA0223-250, ATGA0223-500, ATGA0223-01M

References & Citations

De Vries L., et al. (1998) . Proc Natl Acad Sci U S A 95 (21) : 12340-5.; ; Liu T F, Kandala G, Setaluri V (2001) . J. Biol. Chem. (United States) 276 (38) : 35768-77.; ; Hu, Liaoyuan A., et al. (2003) . J. Biol. Chem. (United States) 278 (28) : 26295-301.;

Other References

Wang Q, et al. Modulation of m-Opioid Receptor Signaling by RGS19 in SH-SY5Y Cells. (Mol Pharmacol. 2013) {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558815/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_060125.4

Uniprot Accession Number

Q8TF65

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: GIPC1 recombinant protein; ; Lane 2.: GIPC2 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: HeLa cell lysate {2004010182665280.jpg}

FACS Description

Flow cytometry analysis of GIPC in HeLa cells. The cell was stained with ATGA0223 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .