Influenza A H1N1 Hemagglutinin antibody

Influenza virus, an enveloped virus of the Orthomyxoviridae family, has a unique capacity for genetic variation that is based in two molecular features of the virus family. First of all, the surface proteins of the virus are highly variable, able to mutate up to 50% of their amino acid sequence and still perform their functions in infection. Secondly, the viral genome is segmented, with eight RNA segments that are genetically independent of one another. In a mixed infection of different influenza genotypes, these segments can almost randomly reassort resulting in hybrid genotypes with some segments derived from one virus strain, while the other segments are derived from a second strain.

Product Specifications

Background

ELISA: H1N1/HA1 Antibody (1 µg/mL) specifically recognizes H1N1 recombinant protein, but not interacted H3N2 and H5N1 recombinant protein in ELISA.{ATGA0221-Addpic.jpg}

Product Name Alternative

Hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA

Host

Mouse

Antigen Species

Influenza A-H1N1, Influenza A

Reactivity

Influenza A

Immunogen

Recombinant Influenza A-H1N1/HA1 (18-344aa) purified from Baculovirus

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1G7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

H1N1, HA1, Influenza, hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA, ATGA0221-10 µg, ATGA0221-20 µg, ATGA0221-50 µg, ATGA0221-100 µg, ATGA0221-250 µg, ATGA0221-500 µg, ATGA0221-1 mg, ATGA0221-10, ATGA0221-20, ATGA0221-50, ATGA0221-100, ATGA0221-250, ATGA0221-500, ATGA0221-1

References & Citations

Kilbourne ED: Influenza pandemics of the 20th century. Emerg Infect Dis 2006,12:9-14. ; ; Russell RJ, et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (46) : 17736-41.; ; AJ. Hay, et al. Philos. Trans. R. Soc. Lond. B Biol. Sci. 356, 1861 (2001) .;

Other References

Sakamoto H, et al. A Novel Optical Biosensing System Using Mach-Zehnder-Type Optical Waveguide for Influenza Virus Detection. (Appl Biochem Biotechnol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26498024/}; ; Shim DH, et al. Development of a HA1-specific enzyme-linked immunosorbent assay against pandemic influenza virus A H1N1. (Clin Exp Vaccine Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/30775353/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Other

NCBI Accession Number

ACV04238.1

Uniprot Accession Number

C7RYS4

WB Description

The recombinant Influenza A-H1N1/HA1 proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A-H1N1/HA1 antibody (1:500) . (1:1000), (1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.