Human Cyclophilin C/PPIC antibody

PPIC is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A.

Product Specifications

Product Name Alternative

Cyclophilin C, CYPC, Peptidyl prolyl cis trans isomerase C, PPIase C, Rotamase C, PPIC

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPIC (39-197aa) protein purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3C6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cyclophilin C, CYPC, Peptidyl prolyl cis trans isomerase C, Peptidyl-prolyl cis-trans isomerase C, PPIase C, Rotamase C, PPIC, ATGA0218-10 µg, ATGA0218-20 µg, ATGA0218-50 µg, ATGA0218-100 µg, ATGA0218-250 µg, ATGA0218-500 µg, ATGA0218-1 mg, ATGA0218-010, ATGA0218-020, ATGA0218-050, ATGA0218-100, ATGA0218-250, ATGA0218-500, ATGA0218-01M

References & Citations

Takahashi, N., et al. (1989) . Nature (London) 337, 473-475.; ; Fischer, G., et al. (1989) . Nature (London) 337, 476-478.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000934

Uniprot Accession Number

P45877

WB Description

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of PPIC in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of PPIC in HeLa cells. The cell was stained with ATGA0218 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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