Human Myoglobin antibody

MB (Myoglobin) is an an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be diagnostically relevant when found in blood.

Product Specifications

Product Name Alternative

Myoglobin, PVALB

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MB (1-154aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6E10

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Myoglobin, ATGA0217-10 µg, ATGA0217-20 µg, ATGA0217-50 µg, ATGA0217-100 µg, ATGA0217-250 µg, ATGA0217-500 µg, ATGA0217-1 mg, ATGA0217-10, ATGA0217-20, ATGA0217-50, ATGA0217-100, ATGA0217-250, ATGA0217-500, ATGA0217-1

References & Citations

George A., et al. (2004) . Journal of Experimental Biology 207 (Pt 20) : 3441-6.; ; Akaboshi E (1985) . Gene 33 (3) : 241-9.; ; J. P. Collman., et al. (2004) . Chem. Rev. 104 (2) : 561-588.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_976312

Uniprot Accession Number

P02144

WB Description

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myoglobin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Muscle Tissue lysate; ; Lane 2.: Mouse Heart Tissue lysate