Human PNMT antibody

Phenylethanolamine N-methyltransferase (PNMT) catalyzes the synthesis of epinephrine and plays an important role in regulating cardiovascular function. Expression of PNMT is regulated by hormonal and neural stimuli because its promoter contains sequences responding to cholinergic and depolarization stimuli. PNMT is the final enzyme in the catecholamine pathway, catalyzing the conversion of norepinephrine to epinephrine using as the cosubstrate and methyl donor, S-adenosylmethionine.

Product Specifications

Product Name Alternative

Phenylethanolamine N-methyltransferase, PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PNMT (1-282aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1C11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PNMT, ATGA0214-10 µg, ATGA0214-20 µg, ATGA0214-50 µg, ATGA0214-100 µg, ATGA0214-250 µg, ATGA0214-500 µg, ATGA0214-1 mg, ATGA0214-10, ATGA0214-20, ATGA0214-50, ATGA0214-100, ATGA0214-250, ATGA0214-500, ATGA0214-1

References & Citations

Wurtman R.J., et al. (1965) Science 150: 1464-1465.; ; Wurtman R.J., et al. (1966) J Biol Chem 241: 2301-2305.; ; Huang C., et al. (2011) lAmerican Journal of Hypertension 24: 1222-1226.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_002677

Uniprot Accession Number

P11086

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: PNMT transfected 293T cell lysate

IF Description

ICC/IF analysis of PNMT in Hep3B cells. The cell was stained with ATGA0214 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .