Human PNPO antibody

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Product Specifications

Product Name Alternative

Pyridoxine-5-phosphate oxidase, FLJ10535, PDXPO

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PNPO (57-261aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2C7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PNPO, ATGA0213-10 µg, ATGA0213-20 µg, ATGA0213-50 µg, ATGA0213-100 µg, ATGA0213-250 µg, ATGA0213-500 µg, ATGA0213-1 mg, ATGA0213-10, ATGA0213-20, ATGA0213-50, ATGA0213-100, ATGA0213-250, ATGA0213-500, ATGA0213-1

References & Citations

Ngo EO, et al. (Jun 1998) . Biochemistry 37 (21) : 7741-8.; ; Mills PB, Surtees RA, et al. (2005) . Hum. Mol. Genet. 14 (8) : 1077-86.; ; Kang JH, et al. (Jun 2004) . Eur J Biochem 271 (12) : 2452-61.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_060599

Uniprot Accession Number

Q9NVS9

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HepG2 cell lysate

IHC description

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PNPO (1:150) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of PNPO in Jurkat cells. The cell was stained with ATGA0213 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PNPO in Hep3B cells. The cell was stained with ATGA0213 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .