Human EGFR antibody

Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.

Product Specifications

Product Name Alternative

Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EGFR (424-605aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2H8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EGFR, ATGA0212-10 µg, ATGA0212-20 µg, ATGA0212-50 µg, ATGA0212-100 µg, ATGA0212-250 µg, ATGA0212-500 µg, ATGA0212-1 mg, ATGA0212-10, ATGA0212-20, ATGA0212-50, ATGA0212-100, ATGA0212-250, ATGA0212-500, ATGA0212-1

References & Citations

Cohen S, (1964) Nat Cancer Inst Monogr, 13: 13-27. ; ; Toyoda H, et al. (1995) J Biol Chem, 270: 7495-500. ; ; Carpenter G, et al. (1978) Nature,276: 409-10.

Other References

Hoshi H, et al. Construction of a novel cell-based assay for the evaluation of anti-EGFR drug efficacy against EGFR mutation. (Oncol Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/27840973/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_005219

Uniprot Accession Number

P00533

WB Description

The cell lysates of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green) .