Human TPMT antibody

TPMT (Thiopurine S-methyltransferase) is a very important enzyme for the metabolizm of thiopurine compounds. TPMT pharmacogenetics has been studied extensively because of its clinical significance. Many patients got benefited from the knowledge of TPMT genotype-phenotype correlation. Also, TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.

Product Specifications

Product Name Alternative

Thiopurine S-methyltransferase, Thiopurine S-methyltransferase, TPMT, Thiopurine S-methyltransferase HGNC:12014, S adenosyl L methionine thiopurine S methyltransferase, Thiopurine methyltransferase, Thiopurine S methyltransferase.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TPMT (1-245aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2E7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TPMT, ATGA0211-10 µg, ATGA0211-20 µg, ATGA0211-50 µg, ATGA0211-100 µg, ATGA0211-250 µg, ATGA0211-500 µg, ATGA0211-1 mg, ATGA0211-10, ATGA0211-20, ATGA0211-50, ATGA0211-100, ATGA0211-250, ATGA0211-500, ATGA0211-1

References & Citations

Milstein O., et al. (2008) J Biol Chem. 283 (49) :34414-22.; ; Aldape MJ., et al. (2007) J Infect Dis. 195 (12) :1838-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000358

Uniprot Accession Number

P51580

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant protein (50ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TPMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant Protein ; ; Lane 2. : HepG2 cell lysate {ATGA0211-WB2.jpg}

IF Description

ICC/IF analysis of TPMT in U87MG cells. The cell was stained with ATGA0211 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .