Human NANS antibody

NANS (Sialic acids synthease) is an enzyme that functions in the biosynthetic pathways of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.

Product Specifications

Product Name Alternative

Sialic acid synthase, SAS, N acetylneuraminate synthase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NANS (1-359aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1G6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NANS, ATGA0210-10 µg, ATGA0210-20 µg, ATGA0210-50 µg, ATGA0210-100 µg, ATGA0210-250 µg, ATGA0210-500 µg, ATGA0210-1 mg, ATGA0210-10, ATGA0210-20, ATGA0210-50, ATGA0210-100, ATGA0210-250, ATGA0210-500, ATGA0210-1

References & Citations

Milstein O., et al. (2008) J Biol Chem. 283 (49) :34414-22.; ; Aldape MJ., et al. (2007) J Infect Dis. 195 (12) :1838-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_061819

Uniprot Accession Number

Q9NR45

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: NANS Transfected 293T cell lysate {ATGA0210-WB2.jpg}

IF Description

ICC/IF analysis of NANS in HeLa cells. The cell was stained with ATGA0210 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .